Alexandra J Zani

Alexandra J Zani

Mature Considerations: Properly Treating & Caring for the Aging Client

The aging process is a natural, biological event that has been genetically programmed into the human species. Additionally, the term aging echoes into the social and economic sectors of everyday life. Viewpoints, including stereotyping, are reflective of the society and geographical region in which an individual resides. These perceptions are quickly shifting as an era of new perspectives emerges, which allows for extraordinary individual transformation. Most significantly, maturing clients are remaining healthier and vital throughout their life passages of 50 and beyond. They may be striving for retirement, reprioritizing their life and work, and adjusting to the new normal established during this past year. Indeed, 2020 provided opportunities of profound insight in how skin care professionals are going to best serve clients.

Gail Sheehy, author of the of the classic bestseller, “Passages,” compared the passage of life as being analogous to a lobster which grows by developing a series of hard, protective shells. Each time it expands from within, it must shed a confining shell. In parallel, in each passage from one stage of adult life to another, one must shed some of their protective structure, which leaves them temporarily exposed and vulnerable. During this process, however, one also becomes embryonic and exuberant again. A heightened potential appears to stretch towards new growth. Throughout this aging process, one may feel panicky and retreat or ignore the impulse to change and remain stuck in their shells. No matter the choices made, the future will be rendered better or worse, but in any case, restructured.

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Alexandra ZaniAlexandra J. Zani is an international educator, licensed instructor, speaker, author, and researcher in the professional skin care industry. Academic background includes cell biology and medical technology. Zani is on the Education Commission of the International Association for Applied Corneotherapy, is a member of NCEA (National Coalition of Estheticians, Associations, and Distributors), and is certified in Oncology Esthetics and the Pastiche Method of Skin Analysis. Zani is the owner and director of AEsthani Skincare Institute, LLC in Greenville, South Carolina and is also co-founder of Intellective Aesthetics, dedicated to post-graduate aesthetics studies.

Cystic Acne Considerations: Pathology & Treatment

Defined as acne grades four and five, cystic acne is a skin condition centered on the pilosebaceous unit that may exhibit intense erythema, inflammation, nodules, cysts, and scarring. It is considered a rare and serious form of acne vulgaris. Acne vulgaris affects approximately 80of adolescents and young adults. Several clinical types of acne also include less inflamed comedones and pseudocysts that may occur at the same time as the more severe lesions. Inflamed lesion results in the rupture of a closed comedone.Acne can affect the face or spread to the neck, chest, and back. The onset of debilitating acne lesions can interfere with the quality of life and self-esteem. 




Acne is a disease of the sebaceous follicle, primarily affecting the face, back, and chest. It is an inflammatory disorder of the pilosebaceous unit (hair follicle), a very active apparatus residing within the dermis and epidermis. There are four main interrelated pathogenic factors involved with this apparatus, including excess and altered sebum production under the control of androgens, abnormal follicular hyperkeratinization and differentiation, follicular canal colonization with cutibacterium acnes, (c. acnes), and chronic inflammation (controlled by a complex innate and acquired immunological mechanisms.)


Nodular Acne

Nodules appear as raised and solid lesions and are located deeper into the dermis as subcutaneous tissue. 


Cystic Acne

Cystic acne is similar to nodules; however, they are filled with pus, appearing large and swollen.



To understand acne, the structure and function of the pilosebaceous unit are importantThe hair follicle is an active apparatus residing within the dermis and epidermis. There are two kinds of folliclesa hair, and a sebaceous follicleBoth follicles have sebaceous glands attached. There may be microscopic hair in the follicle called rudimentary hair. When acne develops, this hair usually coils up, gets lost in the rest of the debris, and never gets to the surface.

Sebum inside the follicle plays an important role in the building of the acid mantle. The environment within the follicle normally contains anaerobic bacteria (grows without oxygen). Bacteria feed off the triglycerides produced by the sebaceous glands, leaving fatty acid by-products. The sebum moves from the sebaceous gland interior and flows up the hair shaft to the surface of the skin.

The stratum corneum curves into the top of the follicle where the opening serves as an exit or entrance to the pilosebaceous duct. Epithelial cells inside the follicle are an extension of the stratum corneum. They are interspersed with melanocytes and line the entire pilosebaceous unit. At the base of the follicle is the papilla that supports the various stages of the hair growth cycle. Also known as the bulge, the papilla houses several types of stem cells that supply the follicle with new cells.

Just as cells desquamate off the stratum corneum, the same process occurs inside the follicle where the movement of sebum washes cellular debris upward to the opening of the follicle, spilling its contents onto the surface of the skin. The hair then acts as a wick inside the follicle.


Role of Cutibacterium

Cutibacteriumor c.acnes (formerly propionibacterium acnes) is a slow-growing, anaerobic bacteria that naturally colonize within the duct of the sebaceous follicle.C. acnes secretes numerous proteins including digestive enzymes. These enzymes are involved with the digestion of sebum and the acquisition of other nutrients. In healthy skin, the bacterium resides within the fatty acids in the sebum secreted by sebaceous glands within the follicle. Sebum lubricates the skin and hair and acts as a seal in the hair follicle, protecting it from the outside. As the sebum rises upwards towards the follicle opening, it moves onto the skin’s surface transforming a moreacidic stateWhen the sebum amalgamates with the epidermal lipids, the skin flora and microbiome at the stratum corneum and acid mantle meet.

The onset of acne causes an innate immune response that initially develops as a microcomedoneHowever, the combination of increased sebum with abnormal hyperproliferation of keratinocytes can easily escalate into enlarged and inflamed lesions as bacteria becomes entrapped within the follicular cavity. The acidic nature of the free fatty acids now becomes pro-inflammatory and comedogenic. Due to the arrival of the cells from the immune system to combat the inflammation and infection, irritation soon causes pustules. The immune system looks at the bacteria components of the c.acnes as adversary molecules. Immune cells also release large amounts of inflammatory cytokines that induce more white blood cells to release destructive enzymes and free radicals at the infection site. The follicle and surrounding tissue become red and swollen with the increased immune response that vigorously combats trapped bacteria. The presence of white blood cells (pus) causes the lesion to expand and finally rupture. The skin’s surface becomes a field of inflammation with swollen lesions and compromised tissue.


Table 1 - Clinical features of acne

There are numerous grades based on severity  mild, moderate, or severe. Comedones and inflammatory lesions are normally considered separately.

Superficial lesions

  • Open and closed comedones (blackheads and whiteheads)
  • Papules (small, tender red bumps)
  • Pustules (white or yellow “squeezable” spots)

Deeper lesions

  • Nodules (large painful red lumps)
  • Pseudocysts (cyst-like fluctuant swellings)

Secondary lesions

  • Excoriations (picked or scratched spots)
  • Erythematous macules (red marks from recently healed spots – best seen in fair skin)
  • Pigmented macules (dark marks from old spots, mostly affecting those with dark skin)
  • Scars or various types.



Recent research confirms that inflammation exists throughout the lifecycle of an acne lesion and even prior to a comedone formation. This statement is significant because skin care professionals must carefully examine their approach for treatment, taking into consideration the inflammatory factor, age, environment and lifestyle, and the overall health profile of thclient. Inflammation is a vigorous indicator that the immune system is involved. Moreover, it is an indicator as to how the skin care professional is going to manage a long-term care pathway.



Hormones are linked to acne from adolescent to adult life. They are responsible for the development of the sebaceous glands that produce sebum. During puberty, the body produces an excess of male hormones (androgens) stimulating an overproduction of sebum. Adolescent acne occurs during early adolescence years during which time hormonal fluctuations accompany puberty transformation into teenage years to adulthood. Normally, acne improves after puberty, but severe cases may leave pitted scarsWhile in other cases, it can linger on into the client’s early 20s. The appearance of these acne lesions accompanies the active life of approximately 70% of teenagers. 

Adult acne may occur due to hormonal changes during a women’s menstrual cycle, pregnancy, and menopause. Drug-induced acne is an adverse effect of a series of systemic drugs such as corticosteroids, thyroid hormones, antibiotics, lithium, halogen compounds, antibiotics, and during chemotherapy treatments. Acne can also become a red flag of an underlying disease, especially if there is no prior history of acne. Examples are polycystic ovarian syndrome or other endocrine disorders.




The role of c.acnesbacteria is a gram-positive, non-spore-formingand normal skin bacterium typical to human skin. It contributes to the formation of the acid mantle and skin barrier. They have evolved to be both anaerobic and aero-tolerant and reside deep within the follicleThey are found at the top of the pilosebaceous duct and on the skin surface near the exit. As sebum moves up the follicle, the triglycerides are transformed into free fatty acids. C.acnes use sebum, cellular debris, and metabolic byproducts as an energy source. They ingest the abundant triglycerides in the sebum and excrete specialized enzymes (lipases) required to create the free fatty acids. 

During normal functioning, c. acnes do not directly cause significant damage to the follicle and surrounding skin. Most of the damage caused by acne is due to an inflammatory process of the immune system.9Whenever there is an impairment of the acid mantle compounded with follicular imbalance the c.acnes moves away from their normal environment and seeks the next highest source of triglycerides – the sebaceous gland interior down inside the follicle. It is here where the bacteria continue to breakdown triglycerides into free fatty acids. Unfortunately, this location is very close to the dermis that has a pH of around seven. The acidic nature of the free fatty acids now becomes pro-inflammatory and comedogenic. Due to the arrival of the cells from the immune system to combat the inflammation and infection, the irritation soon causes nodules or pustules. The immune system looks at the bacteria components of the c. acnes as adversary molecules. Immune cells release large amounts of inflammatory cytokines that induce more white blood cells to release destructive enzymes and free radicals at the infection site.

Some individuals with acne vulgaris have a faulty and oversensitive immune response that causes the arrival of more inflammatory cytokines to induce additional white blood cells that continue to fight infection. This vicious cycle begins to affect the surrounding tissue with more injury, continuing the propagation of bacteria. Furthermore, it is not uncommon to have ruptured glands that form fibrotic tissue. They can be shallow or deep, creating permanent pitting and scarring.



A genetic predisposition in the makeup of the follicle structure may occur during embryonic development. Some individuals may inherit more sebaceous glands on the face and other areas or have inherited a higher sensitivity to circulating androgens. A hereditary factor called retention hyperkeratosis may be present, whereby the keratinocytes do not shed off the surface on the stratum corneum and out of the follicle as in normal skin.



Androgens are hormones that are important for normal male sexual development during embryonic development and during puberty. The AR gene provides instructions for making a protein called the androgen receptor. Androgens and their receptors direct the development of male sexual characteristics. They also have important functions in both males and females such as hair growth and sex drive.10

Androgen receptors located within the sebaceous follicle stimulate an upsurge of sebum production. At the onset of puberty, androgen hormones are produced, beginning with testosterone. An enzyme called 5-alpha-reductase converts testosterone to dihydrotestosterone. Dihydrotestosterone stimulates the sebaceous glands to begin the production of sebum.

Retention hyperkeratosis is a result of the buildup of epithelial cells. Continuous overproduction of sebum causes distention of the gland and a notable enlargement of the follicle. Essentially, the process of pimple formation is a result of an obstruction in the follicle. Cellular debris mixes with sebum dry out and creates a plug that obstructs the drainage of the sebum.

Other circumstances that affect and modulate sebum production include pregnancythe use of oral contraceptiveslow levels of circulating androgens in womenhormonal changes during menopause, and drugs.



Acne is an inflammatory condition involving the activity of the hair follicle, the immune system, as well as fluctuating hormone activity. Moreover, the skin barrier has been compromised due to breakout, pustules, and over-activity of bacteria. The stratum corneum is a biologically active cellular tissue and requires a pathway of restoration based on corneotherapy principles for the correction of skin conditionsThe improper choice of treatment and products may irritate, cause more inflammation, and prevent the restoration of the skin. A significant objective is to obtain a clear understanding of the nature of acne and what stimulates its onset and what calms it. Age, health profile, diet, and lifestyle are fundamental when determining a treatment pathway.

There are effective options for remedying acne, including supporting the skin barrier through in-clinic and homecare intervention using formulations that provide a balancing, restorative, and anti-inflammatory effect to the skin. Lymphatic drainage, photo modulation, microcurrent, and other less invasive interventions may become necessary to support a reduction in inflammation and gradual correction. It is highly recommended to reduce stress, modify lifestyle habits that may not be supportive, like smoking, and poor diet and health care.

During acute inflammation, a primary focus is to reduce the swelling and infection and support the well-being of the client. Improvement of the health of the acid mantle is essential. A multi-faceted approach may be recommended, especially when it comes to conditions beyond the scope of practice for the skin care professional. Teaming with a medical professional may be necessary to add a medical component that addresses the severity of the condition. There also may be nutritional deficiencies such as vitamin D, essential fatty acids, and other nutrients that can be evaluated and adjusted by a licensed professional.

Skin care ingredients for improving acne may contain active agents with attributes that are sebum-liquefying, sebum-suppressive, keratosis-impeding, antimicrobial, anti-inflammatory immune-suppressive, and regenerativeFor example, studies with the application of liposomal phosphatidylcholine showed improvement of acne vulgaris comedones after 14 to 28 days. Use caution when choosing the appropriate composition of base creams and cosmetic products in order to avoid counterproductive effects. Cleansers and active ingredients should not dry out, stripor irritate.Skin care regimens require review throughout the course of correction and should be adjusted when necessary.


Examples of Active Agents: Selection is dependent upon the acne type.

Keratolytic effects

Salicylic acid and willow bark extracts, urea, alpha hydroxy fruit acids (only to be used if required and not as a routine), enzyme peelings, and azelaic acid

Anti-inflammatory effects

Boswellia acids are protease inhibitors. The dermal 15-liposygenase transfers of essential fatty acids of herbal oils, such as linoleic acids, alpha-gamma-linoleic acids can transfer into anti-inflammatory metabolites.

Phosphatidyl-choline, buckhorn extract, chamomile, hamamelis (witch hazel), green teaand zinc


Azelaic acid (5-alpha-reductase inhibitor, algae extract, betulinic acid (from the bark of the Betula alba, (white birch), and benzoyl peroxide

Regenerative - supporting

Vitamin A and derivatives, niacinamide (Vitamin B3), yeast extracts (B vitamins), D-panthenol (provitamin B5), sodium ascorbyl phosphate (vitamin C), vitamin E and esters, echinacea (coneflower, sunbonnet) extract, and phytohormones (soya, red clover) with their high content of isoflavonoids.




  1. Marissa D. Newman, et al. Therapeutic considerations for severe nodular acne. Am J Clin Dermatology, Feb. 2011;12(1)
  2. What is Cystic Acne? Retrieved from
  3. Tanghetti, Emil A., MD.The Role of Inflammation in the Pathology of Acne.Clinical and Aesthetic Dermatology.Retrieved from
  4. What are inflammatory lesions in acne? Dr. Amanda Oakley, Dermatologist.Retrieved from
  5. McLaughlin, Joseph, et al.Propionibacterium acnes and Acne Vulgaris: New Insights from the Integration of Population Genetic, Multi-Omic, Biochemical, and Host-Microbe Studies.Https://
  6. Zani, A. (June 2015). The Pathogenesis of Acne.Retrieved from
  7. Grice, E.A., The skin microbiome.Retrieved from
  8. What are Propionibacterium acnes?Retrieved from
  9. Acne vulgaris.Reviewed and updated by Dr. Amanda Oakley Dermatologist, Hamilton, New Zealand, June 2014
  10. IBID See Ref. 3
  11. Harvard Blog.Adult acne: Understanding underlying causes and banishing breakouts.Retrieve from

1 What is Corneotherapy.

  1. Lautenschlager, Dr. Hans, Elke Klein.KosmetikInternational 2003 (5), 27-31. Retrieved from
  2. IBID See Ref 3
  3. Lautenschlager, Dr. Hans.Hormones control puberty, pregnancy, and menopause - can we control their impact? Retrieved from



How Long Does It Take for Products to Work

There are several factors to consider when applying topical products to the skin. It is also important to take into consideration the focus of a product and its relationship to the skin – in essence, what are the concerns? How quickly it may or may not work may be dependent on the efficacy of the product and the bioavailability of its active ingredients. Moreover, an essential and number one consideration involves the client and their age, health, and lifetime habits. The success of any change is dependent upon compliance to usage combined with a realistic awareness of what results are possible given the state of their skin. Too often, clients try a product touting big promises and use it for a week or two, only to be disappointed after they don’t see any significant improvements.


A 15th Century adage written by playwright John Heywood sums up the answer regarding how quickly a product works on the skin: “Rome wasn’t built in a day, but they were laying bricks every hour.”


When a client comes to me after a lifetime of environmental exposure, poor nutrition, or smoking, requesting a transformation in their skin, a moment of truth ensues. Restoring the appearance of the skin begins with evaluating the actual biological age of the cells and systems within the skin, including the condition of the barrier function. What is a realistic time frame for change? What is truthfully possible? What is their commitment?


Predicting how fast the skin can heal is an unknown until you begin a consistent pathway for its correction. In my practice, I invite a client to partake in a five-week bootcamp, if their time allows. Twice weekly, they receive a facial treatment that includes professional products and also microcurrent. The client’s home regimen is carefully designed to be simple and not overwhelming. The chosen products will work in tandem with the in-clinic work. There is an accumulative affect that begins to manifest in visual changes during the first and second weeks. At each return visit, there may be more improvement. Realistically, however, change takes place over time, one cell (brick) at a time. This timeline is also dependent upon age and the client’s ability to heal and be compliant. The body heals on its own time, including improving the health of the skin. Beauty begins from within. Promises of instant gratification with a product is unrealistic.


Alexandria ZaniAlexandra J. Zani, licensed instructor, owner of AEsthani Skincare Institute, LLC, is an award-winning international educator, researcher, and author with a background in cell biology and medical technology. Her passion for education resulted in earning numerous advanced certifications, both in the United States and abroad. Zani holds an instructor license for aesthetics and cosmetology, is certified in oncology aesthetics, and the pastiche method of Advanced Skin Analysis, and is a member of NCEA. She is on the education committee for the International Association of Applied Corneotherapy (IAC) and is co-founder of Intellective Aesthetics.




The Inflammatory Story: Two Disorders with Similar Origins and Distinct Differences

What is the difference between acne and rosacea? How can professionals accurately identify each condition? Both manifest visual signs that require further exploration into the understanding of the pathophysiology of each disorder. Some general similarities are present in both conditions due to the innate immune response that triggers inflammation. Moreover, acne and rosacea may share a common presence of papules on the face. The differences between each, however, are greater than their similarities.


The skin becomes compromised whenever there are conditions that interfere with its normal function. Inflammation often erupts when the immune system is triggered, whether from internal or external influences. Moreover, the skin is further aggravated by numerous triggers, including allergens, disease, poor quality skin care, poor dietary habits, and environmental influences. More severe symptoms may even interfere with quality of life. Care of these conditions is multidimensional.

Fact or Fiction: Sleep affects the skin.

Sleep deficiency is reported to be a common health problem in the United States. There are long- and short-term consequences that include heart disease, high blood pressure, kidney disease, diabetes, stroke, obesity, and depression.1 Physical signs may be swollen, sunken eyes, dark circles, pale dehydrated skin, and barrier dysfunction.2 Subclinical effects can affect wound healing, collagen growth, skin hydration, and skin texture.3 Moreover, there is a higher level of inflammation in sleep-deprived individuals that may result in outbreaks of acne, eczema, psoriasis, and skin allergies.

An In-Depth Look at How Products Permeate the Skin

The chemistry for understanding delivery pathways for the application of topical skin care products is found in the pharmaceutical industry. Cosmetic chemistry follows this model when compounding for transdermal application of a product. Drugs (pharmaceutics) and cosmetics have similar routes for transdermal delivery. It is essential for professionals to have an understanding of the physiology of the skin and how a formulation enters the epidermis.

Managing the Skin During Menopause

Menopause is defined as the point in time when a female’s menstrual cycles permanently cease due to the natural depletion of ovarian follicles/oocytes from aging. During a woman’s cycle, the ovaries release a mature egg called an ovum (oocyte). The onset of menopause displays biological changes within the entire endocrine system, in particular with decreased levels of reproductive hormones, especially estrogen and testosterone.

Adjuvant Therapies for Pre- and Post Surgical Care

The field of pre- and post-operative care in cosmetic surgery began in the early 1990s during a time in which the concept of integrative aesthetics services into a medical practice was a fledgling idea.

The Pathophysiology of Dehydration

Dehydration reveals itself through numerous body signals, such as thirst, dizziness, and low energy. For example, skin may feel parched or dry after hiking or spending time in the hot sun. Relative humidity within a person's environment greatly influences the balance of skin moisture. For example, clients may live in a seasonal climate zone or in a region where homes are heated in the cooler months or air conditioned during the summer. These variables may affect how the skin feels, as well as interfere with its ability to have good moisture balance.

The Significance and History of Skin Color Adaptations

In a transient, fast-paced, technological, and diverse world, skin care professionals interact with numerous skin types and conditions that have multiple influences, including genetic traits, lifestyle, and current state of health. Professionals must be prepared for the sensitivities and expectations arising from clients and remain mindful of how technologies interact with skin cells.

The Physiology of Aesthetic Technology and How It Influences the Skin

The choice and integration of modalities for the treatment of various skin conditions have expanded during the past several years. There is a plethora of electrotherapy devices,1 as well as chemical peels and advanced-cosmeceutical chemistry, that offer cosmetic improvements for the face and body and can be found in both medical and spa environments. The level of use for each modality varies greatly, depending upon its purpose, degree of intrusiveness, and the environment in which it is being performed.

The Business of the Skin

Arecent conversation with a skin care professional revealed that she had concerns regarding her skin care practice. She expressed that she had difficulty rebooking clients. Many came once, but never called back for another appointment. As incentives, she offered discounts or gave away facial gift certificates to local fundraisers. Moreover, she had challenges meeting her monthly expenses. There were a litany of reasons as to why she felt she had failed. If this scenario sounds familiar, perhaps it is time to re-examine your goals and how to best build and service your clients. All stories have great underlying insight.

The Pathogenesis of Acne

A simple definition of acne is that it is a disease of the sebaceous follicle, primarily affecting the face, back, and chest. It is caused by an inflammation of the oil glands that varies in severity, depending upon how much obstruction exists within the follicle.The challenge of acne emerges during a period in life when the first recipients of this outbreak are normally growing teenagers.

More Than Skin Deep | How the skin protects against disorders

There is a great correlation between the morphology of a skin condition and what is chosen for correction in both treatments and the cosmetic chemistry found in product lines. At times, there may be a great deal of emphasis placed on a brand or isolated ingredients without realizing that a manifested skin condition requires research and contemplation going beyond what is visually apparent. Products are directly formulated to impact the skin. A key factor is to realize that the skin care professional should perform a thorough skin analysis, following an intelligent pathway that leads to discovering the underlying cause of a skin condition. This information becomes a mainstay as we continue to build the ideal skin correction program for clients.

Knowing Your Boundaries

In a society that seeks an instant fountain of youth in a pot, ingredient or treatment, an essential role for the aesthetician is to be able to understand and choose professional products and treatments that are safe and appropriate for each client. How does the aesthetician decipher the hundreds of product ingredients in a chosen skin care line? It stands to reason that many of us become perplexed as to what product/ingredients will best serve our client. Are the ingredients compatible with your client’s skin? Will they truly produce a result? A greater picture here is the ability to link a skin condition to suitable ingredients that elicit optimum correction without causing an immune response that further compromises the client’s skin.

Gradation of Skin Color: Biological Considerations During Treatment

Laser hair removal procedures in 2010 totaled to 936, 121, which reflected a -26.9% decrease from 2009 – most likely as a result of a flexing economy.1 It is a procedure, however, that continues to be popular.
Lasers (including the CO2 laser) were introduced in dermatology and surgery as early as the 1960s.2 The CO2 laser became more popular during the 1990s for skin resurfacing to improve wrinkles, dischromias, scars, atrophic scars, pitted acne scars and others.3 These pioneering procedures also meant longer recovery periods.

October 2021

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  • Alexandria Professional
  • Celluma by Biophotas, Inc
  • DMK Skin Revision Center